Make a Spore Print

Create a piece of art with your mushroom!


In this activity, you are going to collect the spores of your mushroom in a spore print. Making a spore print is a fun way to learn how a mushroom propagates (spreads its seeds) and a creative way to make mushroom art.


What is a spore? A spore is the seed of the fungus and is the means with which the fungus reproduces. Mushrooms, the fruiting bodies of fungi, carry millions of spores in a wide variety of ways, some of which include: on the underside of the mushroom cap on gills or in tubes, on spines, pores, or wrinkled surfaces of the mushroom, enclosed in fruiting bodies (puffballs) or in a saclike structure (flask, cup, brain or sponge fungi).

The mushrooms grown from your kit (elm oyster, blue oyster, shiitake, etc.) all carry their spores in gills on the underside of their caps. When the mushroom ages, it begins to drop its spores. The spores generally drop close to the mushroom, but can be carried to other places by the wind or by animals. Because each spore carries 50% of the mycelium’s DNA, it must combine with another compatible spore for healthy, fertile mycelium to grow.

Materials Needed:


  1. Find a place in the house that you can set up your spore print so that it can sit undisturbed. Once you set up, its best not to move the spore print.
  2. Cut the caps off of the stems of your mature mushrooms (ask a parent for permission first before using a knife).
  3. Place the caps, gills facing down, on the appropriate colored paper. Use dark paper if you are using an elm oyster, blue oyster or shiitake mushroom (which will drop white spores) and a light or white paper if you are using a pioppino mushroom (which will drop brown spores).
  4. Cover the mushrooms with a bowl or cups so they don’t dry out.
  5. Let the sporulating mushroom sit, undisturbed, for at least 12 hours.
  6. Carefully lift the cups or bowls off of the mushrooms and behold! You should see a beautiful spore print of your mushroom. If you do not see a spore print yet, place the mushroom back on the paper and wait another 12 hours.
  7. If you wish to keep your spore print, spray it with hair spray or aerosol fixative.
  8. Move your mushroom to a different spot on the same page or use a new page and repeat the process. You should be able to create additional prints; sometimes up to 5-6 prints over the course of 5-7 days. Your mushrooms should be composted after about a week.